Sigma Tris-Borate-EDTA buffer, 5x concentrate

Tris-Borate-EDTA buffer

T4415-1L

BioReagent, suitable for electrophoresis, 10× concentrate

Application

Ready for use in gel electrophoresis after dilution to working concentrations.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. 
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Other Notes

TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

Packaging

The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.

Preparation Note

Prepared with 18 megohm water

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).